Les concentrations de gastrine srique taient significativement augmentes chez les malades en comparaison des tmoins. gastro-intestinale et 15 chiens malades atteints dentrite lymphocytaire-plasmocytaire chronique ont t utiliss. Les concentrations de gastrine srique taient significativement augmentes chez les malades en comparaison des tmoins. Il y avait galement une corrlation positive entre la svrit des lsions gastriques et la concentration de gastrine srique. Nos rsultats indiquent la possibilit dune implication de la gastrine dans ltiologie de la gastrite chronique de lantre du pylore qui accompagne lentrite lymphocytaire-plasmocytaire chronique canine. = 5), and group B dogs with chronic lymphocytic-plasmacytic enteritis (= 15). Dogs in group A were symptom free and came from owners who voluntarily consented to collaborate in the study. Dogs in group B experienced gastrointestinal indicators (Table 1). All dogs from both groups showed up between January and May 2003 at the Veterinary SU-5402 Medicine Teaching Hospital (VMTH) of the University or college of Madrid. Table 1 Clinical indicators of dogs included in this study (group A dogs without gastrointestinal disease, and group B dogs with chronic lymphocytic-plasmacytic enteritis) for 10 min. Serum was removed and frozen at ?5oC for further analysis. Serum gastrin concentrations were measured by radioimmunoassay, using a commercially available kit (Gastrin J-125 RIA kit; Aurica DRG Diagnostics, DRG Devices GmbH, Marburg, Germany). The assay is usually validated for the species, and samples were assayed in duplicate. Mean gastrin concentrations were used in this study. Briefly, the assay process was as follows: 200 L of gastrin standard (0, 15, 25, 50, 100, 200, 500, and 1000 ng/L) or serum sample was incubated with 100 L of gastrin tracer answer Rabbit Polyclonal to AKAP10 (Gastrin 125J; Aurica DRG Diagnostics) and 100 L of gastrin antiserum (rabbit anti-human gastrin) for 120 min at room heat. The 100 L of tracer was dispensed to only tubes 1 and 2. Afterwards, 1.0 mL of precipitating antiserum was added to all tubes, except 1 and 2, and thoroughly mixed. All tubes, except 1 and 2, were centrifuged at 1500 for 15 min. Supernatants were aspirated from all tubes, except 1 and 2, and radioactivity of the precipitates was measured in each tube by counting in a gamma counter for 1 min. Concentrations of gastrin in serum of dogs were determined by interpolation from the standard curve of % trace binding versus ng/L gastrin. The Wilcoxon test and nonparametric analysis of variance (ANOVA) were used for statistical analysis of the results (statistical program 4.16. Med Calc; MedCalc Software, Mariakerke, Belgium). Significance was considered at 0.05. Results Diagnostic evaluation No abnormal clinical signs or abnormalities on physical examination were evident in the group A dogs throughout the study. On the other hand, a variety of clinical signs relating to the gastrointestinal tract were observed in dogs with chronic lymphocytic-plasmacytic enteritis; the main clinical findings were vomitus (13/15) and diarrhea (9/15). The results of the hematological analysis and biochemical profile were within reference ranges, the results of fecal examination for cestodes, nematodes, and protozoa were negative, and values of fecal chymotrypsin and serum TLI were within reference ranges for all dogs in the study. No abnormalities were observed on the endoscopic exploration or the histological evaluation of the biopsies obtained from group A dogs. On the other hand, in all group B dogs, abnormalities were observed on endoscopic exploration and histological evaluation (Table 2). Gastric lesions located in the pyloric antrum were categorized as follows: absence (5/20), moderate (9/20), and severe (6/20). The duodenal histological lesions were categorized as moderate in all dogs (15/15). Table 2 Gross endoscopic and histopathological findings (stomach and duodenum) in all dogs included in this study (group A dogs without gastrointestinal disease, and group B dogs with chronic lymphocytic-plasmacytic enteritis) = 3.65 ng/L. Mean serum gastrin value for group B dogs was 40.62, = 4.36 ng/L. Significant difference between both groups was observed after statistical analysis (Wilcoxon test); serum gastrin values were significantly elevated in.However, most reports concerning serum gastrin concentrations in humans with inflammatory bowel disease refer to colonic inflammatory bowel disease. All dogs with chronic lymphocytic-plasmacytic enteritis included in this study developed moderate to severe lesions located in the pyloric antrum. que les ventuelles relations avec la svrit des lsions prsentes dans lestomac. Dans ce but, 5 chiens tmoins sans maladie gastro-intestinale et 15 chiens malades atteints dentrite lymphocytaire-plasmocytaire chronique ont t utiliss. Les concentrations de gastrine srique taient significativement augmentes chez les malades en comparaison des tmoins. Il y avait galement une corrlation positive entre la svrit des lsions gastriques et la concentration de gastrine srique. Nos rsultats indiquent la possibilit dune implication de la gastrine dans ltiologie de la gastrite chronique de lantre du pylore qui accompagne lentrite lymphocytaire-plasmocytaire chronique canine. = 5), and group B dogs with chronic lymphocytic-plasmacytic enteritis (= 15). Dogs in group A were symptom free and came from owners who voluntarily consented to collaborate in the study. Dogs in group B had gastrointestinal signs (Table 1). All dogs from both groups arrived between January and May 2003 at the Veterinary Medicine Teaching Hospital (VMTH) of the University of Madrid. Table 1 Clinical signs of dogs included in this study (group A dogs without gastrointestinal disease, and group B dogs with chronic lymphocytic-plasmacytic enteritis) for 10 min. Serum was removed and frozen at ?5oC for further analysis. Serum gastrin concentrations were measured by radioimmunoassay, using a commercially available kit (Gastrin J-125 RIA kit; Aurica DRG Diagnostics, DRG Instruments GmbH, Marburg, Germany). The assay is validated for the species, and samples were assayed in duplicate. Mean gastrin concentrations were used in this study. Briefly, the assay procedure was as follows: 200 L of gastrin standard (0, 15, 25, 50, 100, 200, 500, and 1000 ng/L) or serum sample was incubated with 100 L of gastrin tracer solution (Gastrin 125J; Aurica DRG Diagnostics) and 100 L of gastrin antiserum (rabbit anti-human gastrin) for 120 min at room temperature. The 100 L of tracer was dispensed to only tubes 1 and 2. Afterwards, 1.0 mL of precipitating antiserum was added to all SU-5402 tubes, except 1 and 2, and thoroughly mixed. All tubes, except 1 and 2, were centrifuged at 1500 for 15 min. Supernatants were aspirated from all tubes, except 1 and 2, and radioactivity of the precipitates was measured in each tube by counting in a gamma counter for 1 min. Concentrations of gastrin in serum of dogs were determined by interpolation from the standard curve of % trace binding versus ng/L gastrin. The Wilcoxon test and nonparametric analysis of variance (ANOVA) were used for statistical analysis of the results (statistical program 4.16. Med Calc; MedCalc Software, Mariakerke, Belgium). Significance was considered at 0.05. Results Diagnostic evaluation No abnormal clinical signs or abnormalities on physical examination were evident in the group A dogs throughout the study. On the other hand, a variety of clinical signs relating to the gastrointestinal tract were observed in dogs with chronic lymphocytic-plasmacytic enteritis; the main clinical findings were vomitus (13/15) and diarrhea (9/15). The results of the hematological analysis SU-5402 and biochemical profile were within reference ranges, the results of fecal examination for cestodes, nematodes, and protozoa were negative, and values of fecal chymotrypsin and serum TLI were within reference ranges for all dogs in the study. No abnormalities were observed on the endoscopic exploration or the histological evaluation of the biopsies obtained from group A dogs. On the other hand, in all group B dogs, abnormalities were observed on endoscopic exploration and histological evaluation (Table 2). Gastric lesions located in the pyloric antrum were categorized as follows: absence (5/20), moderate (9/20), and severe (6/20). The duodenal histological lesions were categorized as moderate in all dogs (15/15). Table 2 Gross endoscopic and histopathological findings (stomach and duodenum) in all dogs included in this study (group A dogs without gastrointestinal disease, and group B dogs with chronic lymphocytic-plasmacytic enteritis) = 3.65 ng/L. Mean serum gastrin value for group B dogs was 40.62, = 4.36 ng/L. Significant difference between both groups was observed after statistical analysis (Wilcoxon test); serum gastrin values were significantly elevated in group B as compared with group A. These results are summarized in Table 3. Table 3 Mean serum gastrin values (ng/L) in dogs without gastrointestinal disease (group A) and dogs with chronic lymphocytic-plasmacytic enteritis (group B) 0.02 (Wilcoxon test) Open in a separate window Standard error of the mean; Standard deviation Gastric lesion degree and serum gastrin concentrations Nonparametric ANOVA showed significant difference in serum gastrin levels according to degree of the gastric lesion (F = 4.039, = 0.039) (Table 4). Significant differences in serum gastrin values were observed only.