II. p33, were found to have increased synthesis within 30 min, which decreased in 1 h. Transcription inhibitors were used to ascertain if regulation of their expression was transcriptional or translational. To identify these proteins, we used biotinylated lysine during the in vitro translation reaction, and we extracted the biotinylated protein by using streptavidin magnetic beads. The protein product was analyzed by mass spectrometry. p33 was identified as a prohibitin-like protein (BAP37), but the identification of p72 was not found in the databases. The distinct 2-Hydroxybenzyl alcohol up-regulation and down-regulation of their protein expression suggest their tightly controlled regulation during early T cell activation. and 19 6105C6111. [PubMed] [Google Scholar]Bag, J. and Pramanik, S. 1987. Attachment of mRNA to the cytoskeletal framework and translational control of gene expression in rat L6 muscle cells. 65 565C575. [PubMed] [Google Scholar]Coates, P.J., Jamieson, D.J., Smart, K., Prescott, A.R., and Hall, P.A. 1997. The prohibitin family of mitochondrial proteins regulate replicative lifespan. 7 607C610. [PubMed] [Google Scholar]Collins, J.F. and Crystal, R.G. 1975. Characterization of cell-free synthesis of collagen by lung polysomes in a heterologous system. 250 7332C7342. [PubMed] [Google Scholar]Gygi, S.P., Rochon, Y., Franza, B.R., and Aebersold, R. 1999. Correlation between protein and mRNA abundance in yeast. 19 1720C1730. [PMC free article] [PubMed] [Google Scholar]Heikkila, J.J., Cosgrove, J.W., and Brown, I.R. 1981. Cell-free translation of free and membrane-bound polysomes and polyadenylated mRNA from rabbit brain following administration of d- lysergic acid diethylamide in vivo. 36 1229C1238. [PubMed] [Google Scholar]Jackson, R.J., Campbell, E.A., Herbert, P., and Hunt, T. 1983. The preparation and properties of gel-filtered rabbit-reticulocyte lysate protein-synthesis systems. 131 289C301. [PubMed] [Google Scholar]Jackson, R.J., Hunt, S.L., Reynolds, J.E., and Kaminski, A. 1995. Cap-dependent and cap-independent 2-Hydroxybenzyl alcohol translation: Operational distinctions and mechanistic interpretations. 203 1C29. [PubMed] [Google Scholar]Jagus, R. and Kay, J.E. 1979. Distribution of lymphocyte messenger RNA during stimulation by phytohaemagglutinin. 100 503C510. [PubMed] [Google Scholar]Kecskemethy, N. and Schafer, K.P. 1982. Lectin-induced changes among polyadenylated and non-polyadenylated mRNA in lymphocytes. mRNAs for actin, tubulin and calmodulin respond differently. 126 573C582. [PubMed] [Google Scholar]Kostura, M. and Craig, N. 1986. Treatment of Chinese hamster ovary cells with the transcriptional inhibitor actinomycin D inhibits binding of messenger RNA to ribosomes. 25 6384C6391. [PubMed] [Google Scholar]Kurzchalia, T.V., 2-Hydroxybenzyl alcohol Wiedmann, M., Breter, H., Zimmermann, W., Bauschke, E., and Rapoport, T.A. 1988. tRNA-mediated labelling of proteins with biotin. A nonradioactive method for the detection of cell-free translation products. 172 663C668. [PubMed] [Google Scholar]Lamers, M.C. and Bacher, S. 1997. Prohibitin and prohibitone, ubiquitous and abundant proteins that are reluctant to reveal their real identity. 113 146C149. [PubMed] [Google Scholar]Lee, G.T. and Engelhardt, D.L. 1979. Peptide coding capacity of polysomal and non-polysomal messenger RNA during growth of animal cells. 129 221C233. [PubMed] [Google Scholar]Lockhart, D.J., Dong, H., Byrne, M.C., Follettie, M.T., Gallo, M.V., Chee, M.S., Mittmann, M., Wang, C. Kobayashi, M., Horton, H., et al. 1996. Expression monitoring by hybridization to high-density oligonucleotide arrays. 14 1675C1680. [PubMed] [Google Scholar]Marotta, C.A., Brown, B.A., Strocchi, P., Bird, E.D., and Gilbert, J.M. 1981. In vitro synthesis of human brain proteins including tubulin and actin by purified postmortem polysomes. 36 966C975. [PubMed] [Google Scholar]McCarthy, J.E. and Kollmus, H. 1995. Cytoplasmic mRNA-protein interactions in eukaryotic gene expression. 20 191C197. [PubMed] [Google Scholar]McKeehan, W. and Hardesty, B. 1969. The mechanism of cycloheximide inhibition of protein synthesis in rabbit reticulocytes. 36 625C630. [PubMed] [Google Scholar]Milcarek, C., Price, R., and Penman, S. 1974. The metabolism of a poly(A)? mRNA fraction in HeLa cells. 3 1C10. [PubMed] [Google Scholar]Minich, W.B. and Ovchinnikov, L.P. 1992. Role of cytoplasmic mRNP proteins in translation. 74 477C483. [PubMed] [Google Scholar]Miyamoto, S., Chiorini, J.A., Urcelay,.67 247C256. if regulation of their expression was transcriptional or translational. To identify these proteins, we used biotinylated lysine during the in vitro translation reaction, and we extracted the biotinylated protein by using streptavidin magnetic beads. The protein product was analyzed by mass spectrometry. p33 was identified as a prohibitin-like protein (BAP37), but the identification of p72 was not found in the databases. The distinct up-regulation and down-regulation of their protein expression suggest their tightly controlled regulation during early T cell activation. and 19 6105C6111. [PubMed] [Google Scholar]Bag, J. and Pramanik, S. 1987. Attachment of mRNA to the cytoskeletal framework and translational control of gene expression in rat L6 muscle cells. 65 565C575. [PubMed] [Google Scholar]Coates, P.J., Jamieson, D.J., Smart, K., Prescott, A.R., and Hall, P.A. 1997. The prohibitin family of mitochondrial proteins regulate replicative lifespan. 7 607C610. [PubMed] [Google Scholar]Collins, J.F. and Crystal, R.G. 1975. Characterization of cell-free synthesis of collagen by lung polysomes in a heterologous system. 250 7332C7342. [PubMed] [Google Scholar]Gygi, S.P., Rochon, Y., Franza, B.R., and Aebersold, R. 1999. Correlation between protein and mRNA abundance in yeast. 19 1720C1730. [PMC free article] [PubMed] [Google Scholar]Heikkila, J.J., Cosgrove, J.W., and Brown, I.R. 1981. Cell-free translation of free and membrane-bound polysomes and polyadenylated mRNA from rabbit brain following administration of d- lysergic acid diethylamide in vivo. 36 1229C1238. [PubMed] [Google Scholar]Jackson, R.J., Campbell, E.A., Herbert, P., and 2-Hydroxybenzyl alcohol Hunt, T. 1983. The preparation and properties of gel-filtered rabbit-reticulocyte lysate protein-synthesis systems. 131 289C301. [PubMed] [Google Scholar]Jackson, R.J., Hunt, S.L., Reynolds, J.E., and Kaminski, Rabbit Polyclonal to EPHB1/2/3/4 A. 1995. Cap-dependent and cap-independent translation: Operational distinctions and mechanistic interpretations. 203 1C29. [PubMed] [Google Scholar]Jagus, R. and Kay, J.E. 1979. Distribution of lymphocyte messenger RNA during stimulation by phytohaemagglutinin. 100 503C510. [PubMed] [Google Scholar]Kecskemethy, N. and Schafer, K.P. 1982. Lectin-induced changes among polyadenylated and non-polyadenylated mRNA in lymphocytes. mRNAs for actin, tubulin and calmodulin respond differently. 126 573C582. [PubMed] [Google Scholar]Kostura, M. and Craig, N. 1986. Treatment of Chinese hamster ovary cells with the transcriptional inhibitor actinomycin D inhibits binding of messenger RNA to ribosomes. 25 6384C6391. [PubMed] [Google Scholar]Kurzchalia, T.V., Wiedmann, M., Breter, H., Zimmermann, W., Bauschke, E., and Rapoport, T.A. 1988. tRNA-mediated labelling of proteins with biotin. A nonradioactive method for the detection of cell-free translation products. 172 663C668. [PubMed] [Google Scholar]Lamers, M.C. and Bacher, 2-Hydroxybenzyl alcohol S. 1997. Prohibitin and prohibitone, ubiquitous and abundant proteins that are reluctant to reveal their real identity. 113 146C149. [PubMed] [Google Scholar]Lee, G.T. and Engelhardt, D.L. 1979. Peptide coding capacity of polysomal and non-polysomal messenger RNA during growth of animal cells. 129 221C233. [PubMed] [Google Scholar]Lockhart, D.J., Dong, H., Byrne, M.C., Follettie, M.T., Gallo, M.V., Chee, M.S., Mittmann, M., Wang, C. Kobayashi, M., Horton, H., et al. 1996. Expression monitoring by hybridization to high-density oligonucleotide arrays. 14 1675C1680. [PubMed] [Google Scholar]Marotta, C.A., Brown, B.A., Strocchi, P., Bird, E.D., and Gilbert, J.M. 1981. In vitro synthesis of human brain proteins including tubulin and actin by purified postmortem polysomes. 36 966C975. [PubMed] [Google Scholar]McCarthy, J.E. and Kollmus, H. 1995. Cytoplasmic mRNA-protein interactions in eukaryotic gene expression. 20 191C197. [PubMed] [Google Scholar]McKeehan, W. and Hardesty, B. 1969. The mechanism of cycloheximide inhibition of protein synthesis in rabbit reticulocytes. 36 625C630. [PubMed] [Google Scholar]Milcarek, C., Price, R., and Penman, S. 1974. The metabolism of a poly(A)? mRNA fraction in HeLa cells. 3 1C10. [PubMed] [Google Scholar]Minich, W.B. and Ovchinnikov, L.P. 1992. Role of cytoplasmic mRNP proteins in translation. 74 477C483. [PubMed] [Google Scholar]Miyamoto, S., Chiorini, J.A., Urcelay, E., and Safer, B. 1996. Regulation of gene expression for translation initiation factor eIF-2 alpha: Importance of the 3` untranslated region. 315 791C798. [PMC free article] [PubMed] [Google Scholar]Miyamoto,.