Memory antibody (IgG type) assessment is useful as many healthy individuals in the epidemic area may be asymptomatic but infected and possess a threat for further spread of the computer virus. followed by 26.2% in the age group 30C39 years. Donors in the age group 40C49 years showed antibody positivity of 16.7%, and no antibody-positive donors were found above 50 years of age. COVID-19 IgG positivity was maximum in replacement donors (61.9%) followed Seletalisib (UCB-5857) by family donors (28.6%) and least involuntary donors (0.6%) Blood donors who showed high IgG positivity were Seletalisib (UCB-5857) mainly of labor class. Antibody IgG testing on medical healthcare workers showed 2.3% positivity. The Seletalisib (UCB-5857) healthcare workers who were posted in COVID-19 duties showed 4.8% positivity in the holding area (waiting area with the treatment of patients till their RT PCR report comes) and 5.7% in other COVID-19 areas related to laboratory work. Healthcare workers doing duties in COVID-19 areas showed 2.7% positivity, while those doing duties in non-COVID-19 emergency areas showed a positivity of 2.2%. Conclusion Our study shows that the prevalence of detectable antibodies was low in the general populace in India and many patients were asymptomatic as Seletalisib (UCB-5857) seen in the blood donors, especially the labor class. Maximum exposure was present in young healthy males of labor class who remained asymptomatic. The healthcare workers were more exposed to COVID-19 as compared to the general populace probably due to lack of precaution and awareness. Those doing non-COVID-19 duties were also uncovered appreciably and needed to take all the precautions required for COVID-19 duties. 1. Background and Objectives SARS-CoV-2 computer virus has caused a global pandemic as declared by the WHO in March 2020. In India, the first case was reported in Kerala around the 30th of January, and since then, many says are active but some are showing flattening [1]. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) is being considered as the gold standard technique that confirms the presence of COVID-19 [2]. However, testing of specimens from multiple sites may improve the sensitivity and reduce false-negative test results [3]. Due to the high infectivity rate and a majority of patients being asymptomatic, widespread serological screening was required. Memory antibody (IgG type) assessment is useful as many healthy individuals in the epidemic area may be asymptomatic but infected and possess a threat for further spread of the computer virus. Monitoring IgG will in turn help us for guiding future decisions on the right time to relax social distancing steps and minimize possible epidemic outbreaks [4]. The role of SARS-CoV-2 IgG antibodies, verified by PCR, has shown positive results for confirming the infection [5]. Both SARS-CoV and MERS-CoV infections were believed to originate in bats, and these were transmitted directly to humans [6]. Following the seroprevalence testing in healthy blood donors, we can monitor the spread of the computer virus among healthy people and, thus, will eventually lead to implementing strategies to reduce the spread. Evidence is also emerging that monocyte/macrophage dysfunction may be central to the immunopathology [7] and that the functional characteristics of antibodies to SARS\CoV\2 spike protein (SP) might be a determinant of disease outcome. Antibody response against enveloped viruses such as SARS\CoV\2 comprises immunoglobulin (Ig) M, IgG3, IgG1, and IgA antibodies to glycoproteins of the computer virus envelope and nucleoproteins (NP, internal to the envelope). IgG (IgG3 and IgG1) antibodies against computer virus envelope glycoproteins have many functional characteristics that make up the most efficacious antibody response against viruses, as illustrated by human immunodeficiency computer virus- (HIV-) 1 contamination [8]. Healthcare workers are the frontline workforces who provide care to both suspected as well as confirmed COVID-19 cases. Thus, they are at a higher risk of acquiring the disease, and if they are infected, they possess a serious risk for vulnerable patients as well as fellow healthcare workers. Thus, a need was felt to conduct a study Ptprc to assess the IgG antibody status in healthcare workers differentiating those who were in COVID-19 as well as others in non-COVID-19 emergency duties during this pandemic. Antibodies in healthcare.